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¹ÙÀÌ¿À :: Á¦ÇÑ »çÇ× :: È¿¼Ò´Â ´ÜÀÏ Á¦ÇÑ endonuclease (ƯÁ¤ À§Ä¡¿¡¼­ DNA¸¦ ÀÚ¸£½Ê½Ã¿À).
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  • Rating:
  • ƯÇã:
  • Perl Artistic License
  • °¡°Ý:
  • FREE
  • °Ô½ÃÀÚ À̸§:
  • Bio::Restriction::Enzyme Team
  • °Ô½ÃÀÚ À¥»çÀÌÆ®:
  • http://search.cpan.org/~birney/bioperl-1.4/Bio/Restriction/Enzyme.pm

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Bio :: Restriction :: È¿¼Ò´Â ´ÜÀÏ Á¦ÇÑ endonuclease (ƯÁ¤ À§Ä¡¿¡¼­ DNA¸¦ ÀÚ¸£½Ê½Ã¿À). Bio :: Restriction :: Enzyme´Â ´ÜÀÏ Á¦ÇÑ Endonuclease (ƯÁ¤ À§Ä¡¿¡¼­ DNA¸¦ ÀÚ¸£½Ê½Ã¿À) .Snopsis # ´ÜÀÏ Á¦ÇÑ È¿¼Ò¸¦ ¼³Á¤ÇϽʽÿÀ. ¿©±â¿¡´Â # Rebase ÆÄÀÏ¿¡¼­ ÀϹÝÀûÀ¸·Î ±¸¹® ºÐ¼® µÈ È¿¼Ò¿¡ ´ëÇÑ # Á¤º¸°¡ ¸¹ÀÌ Æ÷ÇԵǾî ÀÖÀ¸¹Ç·Î ´Ù½Ã ÀÐÀ» ¼ö ÀÖ½À´Ï´Ù. Bio :: Restriction :: Enzyme; # ÄÆ ½ÃÄö½º¿Í ÇÔ²² »õ·Î¿î È¿¼Ò¸¦ Á¤ÀÇÇϽʽÿÀ. My $ Re = New Bio :: Restriction :: Enzyme (-enzyme => 'EcoRi', -sq => 'G ^ Aattc'); # ½ÃÄö½º°¡ Á¤ÀÇµÇ¸é ¸î ¹øÀÇ ¹°°ÇÀÌ °è»êµË´Ï´Ù. #### ÇÁ¸® ¼¿·ºÆ® # È¿¼Ò°¡ »è°¨ µÈ °÷À» ã½À´Ï´Ù ... MY $ CA = $ RE-> ÄÆ; # ... ±×¸®°í ±×°ÍÀÌ ¹Ý´ë °¡´Ú¿¡¼­ ÀÚ¸£´Â °÷Àº ³» $ OCA = $ RE-> º¸¿ÏÀûÀÎ ÀÚÈ­; # Àß¶ó ³»±â ¼ø¼­ ¹®ÀÚ¿­À» ´Ù½Ã °¡Á®¿É´Ï´Ù. # »çÀÌÆ®´Â Caret My $ with_caret = $ Re-> »çÀÌÆ®·Î ¼ø¼­¸¦ ¹ÝȯÇÕ´Ï´Ù. #RETURES 'G ^ AATTC'; # ±×·¯³ª ±×°ÍÀº ¶ÇÇÑ ¹ÙÀÌ¿À :: primarySeq °´Ã¼ÀÔ´Ï´Ù .... ³» $ n $ nift_caret = $ Re-> ¼­¿­; # 'gaattc'¸¦ ¹ÝȯÇÕ´Ï´Ù. # ...À̹ǷΠString $´Â $ RE-> ¹®ÀÚ¿­À» Á¦°øÇÏÁö ¾Ê½À´Ï´Ù. # 'GAATTC'µî·Ï; # »è°¨ »çÀÌÆ®ÀÇ ¿ª¹æÇâ º¸¼ö´Â ¹«¾ùÀԴϱî $ rc = $ re-> Revcom; # 'gaattc'¸¦ ¹ÝȯÇÕ´Ï´Ù. # Áö±Ý ÀÎ½Ä ±æÀÌ. ´ÙÀ½ µÎ °¡Áö À¯ÇüÀÌ ÀÖ½À´Ï´Ù. # ÀÎ½Ä _length ()´Â Sequence # Cutter () ÄÆ ÁÖÆļöÀÇ ½ÃÄö½º # Ä¿ÅÍ ()ÀÇ ±æÀÌ ³» $ Recog_Length = $ Re-> ÀÎ½Ä _length; #ÀÌ °æ¿ì¿¡ 6 ¹ø ¹ÝȯµÇÁö¸¸ÀÌ °æ¿ì 6 ¹ø ¹ÝȯµÇÁö¸¸ GanntcÀÇ # 4¿Í RGatcy (BSTX2I)¿¡ ´ëÇØ 5 ¹øÀ» ¹ÝȯÇÕ´Ï´Ù! $ RECOG_LENGTH = $ RE-> Ä¿ÅÍ; # alfindrome - ¶È°°Àº Àü´Þ°ú °Å²Ù·Î ³» $ pal = $ re-> palindromic; # ÀÌ°ÍÀº ºÎ¿ï # ½ÃÄö½º ¹«µòÀÔ´Ï´Ù (Áï, ¿À¹öÇà - ¾ÕÀ¸·Î ³ª¾Æ°¡´Â °ÍÀº µ¿ÀÏÇÕ´Ï´Ù. # overhangÀº ¼¼ °¡Áö °ªÀ» °¡Áú ¼ö ÀÖ½À´Ï´Ù : "5 '", "3'", "µÐ±â", ±×¸®°í Klenow¸¦ »ç¿ëÇÏ´Â °æ¿ì # ¹æÇâÀÌ ¸Å¿ì Áß¿äÇÕ´Ï´Ù! ³» $ OH = $ RE-> ¿À¹öÇà; # ¿À¹öÇà ½ÃÄö½º°¡ ¹«¾ùÀԴϱî? ³» $ OHSEQ = $ RE-> Overhang_Seq; # 'AATT'¸¦ ¹ÝȯÇÕ´Ï´Ù. # ¼ø¼­°¡ ¸ðÈ£ÇÕ´Ï´Ù - ºñ gatc ±âÁö°¡ Æ÷ÇԵǾî ÀÖ½À´Ï±î? MY $ AMBIG = $ RE-> IS_BITOULS; # ÀÌ°ÍÀº ºÎ¿ï Àμâ "enzymencuts¿¡ ´ëÇÑ °ÍÀÔ´Ï´Ù : $ can", "º¸¿Ï Àû ÀÚ¸£±â : $ ocansite : nt $ with_caret orn", "t $ with"; "½ÃÄö½ºÀÇ ¹Ý´ë : $ rcnrecognition ±æÀÌ : $ recog_lengthn", "±×°ÍÀº Palindromic? $ paln"ÀÔ´Ï´Ù. Àμâ "¿À¹öÇàÀº $ OH $ OHÀÔ´Ï´Ù. ### ´ç½ÅÀÌ ¼³Á¤ÇÒ ¼öÀÖ´Â °Í, ºÎÀÚ ÀÎ Rebase ÆÄÀÏ¿¡¼­ ¾òÀ» ¼öÀÖ´Â °Í # isoschizomers¸¦ ¾ò°Å³ª ¼³Á¤ÇϽʽÿÀ (µ¿ÀÏÇÑ # »çÀÌÆ®¸¦ ÀνÄÇÏ´Â È¿¼Ò) $ re-> isoschizomers ( 'pvuii', 'smai'); # Á¤¸»·Î »ç½ÇÀÌ ¾Æ´Õ´Ï´Ù :) Àμâ "Isoschizomers´Â", "", $ re-> isoschizomers, "n"ÀÔ´Ï´Ù. # ¸ÞÆ¿È­ »çÀÌÆ®¸¦ ¾ò°Å³ª ¼³Á¤ÇϽʽÿÀ $ RE-> methylation_sites (2); # Á¤¸»·Î Áø½ÇÇÏÁö´Â ¾Ê½À´Ï´Ù :) "¸ÞÆ¿È­", "", Å°¸¦ °¡ÀÔ ", Å° % {$ RE-> methylation_sites},"n "; # ¶Ç´Â ¼Ò½º ¹Ì»ý¹° $ Re-> Microbe ( 'coli')¸¦ ¼³Á¤ÇϽʽÿÀ. "±×°ÍÀº ¿Ô½À´Ï´Ù", $ RE-> ¹Ì»ý¹° "N"; # IT RE-> ¼Ò½º ( "ROB")¸¦ °í¸³ ½ÃÅ°°Å³ª ¼³Á¤ ÇÑ »ç¶÷À» ¼³Á¤Çϰųª ¼³Á¤ÇÕ´Ï´Ù. # Á¤¸»·Î »ç½ÇÀÌ ¾Æ´Õ´Ï´Ù :) $ re-> source, "USNÀ¸·Î º¸³Â½À´Ï´Ù"; # »ó¾÷ÀûÀ¸·Î ÀÌ¿ë °¡´ÉÇÑÁö ¿©ºÎ¸¦ È®ÀÎÇϰųª ¼³Á¤ÇÏ°í ȸ»ç #Àº $ RE-> °ø±Þ ¾÷ü ( 'NEB')¿¡¼­ ±¸ÀÔÇÒ ¼ö ÀÖ½À´Ï´Ù. # ³»°¡ ÁÁ¾ÆÇÏ´Â Àμâ "´Â »ó¾÷ÀûÀ¸·Î ÀÌ¿ë °¡´ÉÇÕ´Ï´Ù :"; $ RE-> °ø±Þ ¾÷ü¸¦ ÀμâÇϽʽÿÀ. "¿¹ ¾Æ´Ï¿À"; "Àμâ", "°¡ÀÔ", $ RE-> °ø±Þ ¾÷ü, "n"¿¡¼­ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù. # $ RE-> ÂüÁ¶ ( 'Edwards et al. J. Particology')¿¡ ´ëÇÑ ÂüÁ¶¸¦ ¾ò°Å³ª ¼³Á¤ÇϽʽÿÀ. "±×°ÍÀº"$ RE-> ÂüÁ¶ ","N "¿¡ °Ô½ÃµÇÁö ¾Ê¾Ò½À´Ï´Ù. # È¿¼Ò À̸§À» ¾ò°Å³ª ¼³Á¤ÇϽʽÿÀ $ re-> name ( 'bamhi'); "EcoriÀÇ À̸§ÀÌ ½ÇÁ¦·Î", $ re-> name, "n"ÀÌ ¾Æ´Õ´Ï´Ù.ÀÌ ¸ðµâÀº ´ÜÀÏ Á¦ÇÑ ¿£µµ ´º Ŭ·¹¾Æ½º¸¦ Á¤ÀÇÇÕ´Ï´Ù. »ç¿ëÀÚ Á¤ÀÇ Á¦ÇÑ È¿¼Ò¸¦ ¸¸µé±â À§ÇØ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç Bio :: Restriction :: IO°¡ ´º À×±Û·£µå Biolabs Rebase Collection.Use¿¡ È¿¼Ò¸¦ Á¤ÀÇÇÏ´Â µ¥ »ç¿ëµË´Ï´Ù. Bio :: Restriction :: Analysed y any to def ±×µéÀº ´ç½ÅÀÇ ½ÃÄö½º¸¦ ÀÚ¸¨´Ï´Ù. ¿ä±¸ »çÇ× : ¡¤ Perl.


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